af594 conjugated tomato lectin  (Vector Laboratories)

Journal: Theranostics

Article Title: Anti-tumor macrophages activated by ferumoxytol combined or surface-functionalized with the TLR3 agonist poly (I : C) promote melanoma regression

doi: 10.7150/thno.29746

Figure Lengend Snippet: Effect of FMT/PIC combined therapy on tumor blood vessels. C57BL/6 mice were subcutaneously inoculated with 5 × 10 5 B16F10 cells and when the tumors reached an average volume of 100 mm 3 , they were intratumorally treated with PBS, FMT (10 mg/kg), PIC (1 mg/kg), and FMT/PIC (10 mg/kg + 1 mg/kg) (5 per group) every other day for three times. (A) Photograph of dissected tumor tissue covered with vascular tissues from B16F10 tumor-bearing mice with the indicated treatment. (B) LSCM images of blood vessels in tumor sections of mice with the indicated treatment. DAPI, blue. AF594-coupled streptavidin marked lectin, red. (C) Corresponding quantitative percentage of blood vessel area in the total stained area displayed in (B). All representative data are from three independent experiments. Error bars, SD. *P < 0.05, **P < 0.01, ***P < 0.001. DAPI: 4',6-diamidino-2-phenylindole; FCM: flow cytometry; FMT: ferumoxytol; LSCM: laser scanning confocal microscopy; PIC: poly I:C, polyinosinic-polycytidylic acid.

Article Snippet: For angiogenesis staining, mice were intravenously injected with 100 μL AF594-conjugated streptavidin tomato lectin (1 mg/mL in PBS; Vector Labs).

Techniques: Staining, Flow Cytometry, Confocal Microscopy